本科生畢業(yè)論文-酶法制備高麥芽糖漿的研究

哈爾濱商業(yè)大學(xué)畢業(yè)設(shè)計（論文）酶法制備高麥芽糖漿的研究學(xué)生姓名指導(dǎo)教師專業(yè)學(xué)院年月日GRADUATIONPROJECTTHESISHARBINUNIVERSITYOFCOMMERCESTUDYONTHEPRODUCTIONOFHIGHMALTOSESYRUPBYENZYMESTUDENTSUPERVISORSPECIALTYSCHOOL201X0XXX摘要以馬鈴薯淀粉和玉米淀粉為原料，制備高麥芽糖漿。對馬鈴薯淀粉和玉米淀粉的液化情況進行了比較，研究表明馬鈴薯淀粉在液化過程中比玉米淀粉容易被液化酶水解，在較短時間內(nèi)DE值即可達(dá)到實驗要求。采用正交實驗設(shè)計，以耐高溫淀粉酶為液化酶，進行液化，得到了最佳液化條件。關(guān)鍵詞高麥芽糖漿；耐高溫淀粉酶；淀粉酶ABSTRACTUSINGSTARCHANDCORNSTARCH,RESPECTIVELY,HIGHMALTOSESYRUPWASMADETHELIQUEFACTIONOFPOTATOSTARCHANDCORNSTARCHWASCOMPAREDTHERESULTSSHOWEDTHATTHEPOTATOSTARCHCOULDEASILYBEHYDROLYZEDWITHENZYMESTHANCORNSTARCHTHEIDEALDEVALUEWASGOTINSHORTTIMEBYORTHOGONALTEST,THELIQUEFACTIONOFPOTATOSTARCHWASSTUDIEDANDTHEOPTIONALLIQUEFACTIONBYUSINGHEATSTABLEAMYLASEWASOBTAINED，。KEYWORDSHIGHMALTOSESYRUP；HEATSTABLEAMYLASE；AMYLASE目錄摘要IABSTRACTII1緒論111高麥芽糖漿的研究與應(yīng)用1111麥芽糖漿的分類1112高麥芽糖漿的應(yīng)用112高麥芽糖漿國內(nèi)外的研究現(xiàn)狀1121高麥芽糖漿的生產(chǎn)發(fā)展1122馬鈴薯淀粉方面的研究113本課題的來源及研究的意義1131本課題來源1132研究的目的意義12實驗部分221實驗儀器222實驗材料23結(jié)果與討論331單因素實驗3311溫度對淀粉液化的影響3結(jié)論4參考文獻(xiàn)5致謝6附錄71緒論11高麥芽糖漿的研究與應(yīng)用111麥芽糖漿的分類麥芽糖是由2個葡萄糖單位通過1,4糖苷鍵連接而成的二糖，化學(xué)名稱為4OD六環(huán)葡萄糖基D六環(huán)葡萄糖，其結(jié)構(gòu)式見圖11。112高麥芽糖漿的應(yīng)用麥芽糖是一種營養(yǎng)性雙糖，而麥芽糖含量達(dá)60以上的高麥芽糖漿不僅能較好地反映出麥芽糖的性能，還有其獨特的功能特性3。高麥芽糖漿葡萄糖含量低，無色透明，具有甜度低、抗結(jié)晶性好、吸濕性低，并具有良好的熱穩(wěn)定性，因此被廣泛的應(yīng)用于食品、制藥等行業(yè)中。12高麥芽糖漿國內(nèi)外的研究現(xiàn)狀121高麥芽糖漿的生產(chǎn)發(fā)展大約在3000年前，古代中國首先發(fā)明了用麥芽汁水解米中的淀粉生產(chǎn)飴糖的工藝。122馬鈴薯淀粉方面的研究13本課題的來源及研究的意義131本課題來源132研究的目的意義2實驗部分21實驗儀器22實驗材料表21葡萄糖標(biāo)準(zhǔn)溶液配比編號葡萄糖含量/MG葡萄糖液量/ML蒸餾水量/MLDNS液量/ML101010415202020315303030215404040115505050153結(jié)果與討論31單因素實驗311溫度對淀粉液化的影響，結(jié)論以馬鈴薯淀粉為原料，應(yīng)用酶制劑生產(chǎn)高麥芽糖漿的研究，得到以下結(jié)論淀粉的酶解分為液化和糖化兩個過程。液化液的DE值大小影響最后麥芽糖的得率，一般較低DE值可提高麥芽糖產(chǎn)率，但DE值過低，液化不徹底，糊精和低聚糖生成較多，不利于提高麥芽糖的含量和糖化液的過濾及精制。實驗證明，當(dāng)DE值達(dá)10左右時，麥芽糖含量可達(dá)到高麥芽糖漿的生產(chǎn)要求。參考文獻(xiàn)1柴寶華酶法制糖技術(shù)的發(fā)展J淀粉與淀粉糖，2000，2132劉漢文，丁志香，顏洪酶法小麥粗淀粉生產(chǎn)高麥芽糖漿工藝研究J食品科學(xué)1999，20342443黃志蕙高麥芽糖漿的生產(chǎn)與應(yīng)用J中國調(diào)味品1998，28104王志民甘薯淀粉高麥芽糖漿的生產(chǎn)技術(shù)與應(yīng)用J食品科技1998，530315GAOUAROPRODUCTIONOFMALTOSESYRUPBYBIOCONVERSIONOFCASSAVASTARCHINANULTRAFILTRATIONREACTORINDUSTRIALCROPSANDPRODUCTSJ1998，71591676CRAIGS，DAVIDFPHOTOCATALYTICDEGRADATIONCONTAMINANTSMECHANISMSINVOLVINGHYDROXYLRADICALATTACKJJOURNALOFCATALYSIS1990,12211781927張洪薇酶法制備高麥芽糖漿的研究D哈爾濱哈爾濱商業(yè)大學(xué)2004,15208胡國華食品添加劑應(yīng)用基礎(chǔ)M北京化學(xué)工業(yè)出版社2005,1021329陳平,尤宏,羅薇楠等TIO2光催化反應(yīng)器的研究J哈爾濱商業(yè)大學(xué)報2003,192238243致謝感謝導(dǎo)師XXX教授的指導(dǎo)，感謝XXX副教授、XXX在進行畢業(yè)論文工作中所給予的幫助。附錄AGANEMPLOYMENTTRIBUNALCLAIEMLOYMENTTRIBUNALSSORTOUTDISAGREEMENTSBETWEENEMPLOYERSANDEMPLOYEESYOUMAYNEEDTOMAKEACLAIMTOANEMPLOYMENTTRIBUNALIFYOUDONTAGREEWITHTHEDISCIPLINARYACTIONYOUREMPLOYERHASTAKENAGAINSTYOUYOUREMPLOYERDISMISSESYOUANDYOUTHINKTHATYOUHAVEBEENDISMISSEDUNFAIRLYFORMOREINFORMU,TAKEADVICEFROMONEOFTHEORGANISATIONSLISTEDUNDERFURTHERHELPEMPLOYMENTTRIBUNALSARELESSFORMALTHANSOMEOTHERCOURTS,BUTITISSTILLALEGALPROCESSANDYOUWILLNEEDTOGIVEEVIDENCEUNDERANOATHORAFFIRMATIONMOSTPEOPLEFINDMAKINGACLAIMTOANEMPLOYMENTTRIBUNALCHALLENGINGIFYOUARETHINKINGABOUTMAKINGACLAIMTOANEMPLOYMENTTRIBUNAL,YOUSHOULDGETHELPSTRAIGHTAWAYFROMONEOFTHEORGANISATIONSLISTEDUNDERFURTHERHELPATIONABOUTDISMISSALANDUNFAIRDISMISSAL,SEEDISMISSALYOUCANMAKEACLAIMTOANEMPLOYMENTTRIBUNAL,EVENIFYOUHAVENTAPPEALEDAGAINSTTHEDISCIPLINARYACTIONYOUREMPLOYERHASTAKENAGAINSTYOUHOWEVER,IFYOUWINYOURCASE,THETRIBUNALMAYREDUCEANYCOMPENSATIONAWARDEDTOYOUASARESULTOFYOURFAILURETOAPPEALREMEMBERTHATINMOSTCASESYOUMUSTMAKEANAPPLICATIONTOANEMPLOYMENTTRIBUNALWITHINTHREEMONTHSOFTHEDATEWHENTHEEVENTYOUARECOMPLAININGABOUTHAPPENEDIFYOURAPPLICATIONISRECEIVEDAFTERTHISTIMELIMIT,THETRIBUNALWILLNOTUSUALLYACCEPTIIFYOUAREWORRIEDABOUTHOWTHETIMELIMITSAPPLYTOYOUIFYOUAREBEINGREPRESENTEDBYASOLICITORATTHETRIBUNAL,THEYMAYASKYOUTOSIGNANAGREEMENTWHEREYOUPAYTHEIRFEEOUTOFYOURCOMPENSATIONIFYOUWINTHECASETHISISKNOWNASADAMAGESBASEDAGREEMENTINENGLANDANDWALES,YOURSOLICITORCANTCHARGEYOUMORETHAN35OFYOURCOMPENSATIONIFYOUWINTHECASEYOURECLEARABOUTTHETERMSOFTHEAGREEMENTITMIGHTBEBESTTOGETADVICEFROMANEXPERIENCEDADVISER,FOREXAMPLE,ATACITIZENSADVICEBUREAUTOFINDYOURNEARESTCAB,INCLUDINGTHOSETHATGIVEADVICEBYEMAIL,CLICKONNEARESTCABFORMOREINFORMATIONABOUTMAKINGACLAIMTOANEMPLOYMENTTRIBUNAL,SEEEMPLOYMENTTRIBUNALSTHELACKOFAIRUPTHEREWATCHMCAYMANISLANDSBASEDWEBB,THEHEADOFFIFASANTIRACISMTASKFORCE,ISINLONDONFORTHEFOOTBALLASSOCIATIONS150THANNIVERSARYCELEBRATIONSANDWILLATTENDCITYSPREMIERLEAGUEMATCHATCHELSEAONSUNDAY“IAMGOINGTOBEATTHEMATCHTOMORROWANDIHAVEASKEDTOMEETYAYATOURE,“HETOLDBBCSPORT“FORMEITSABOUTHOWHEFELTANDIWOULDLIKETOSPEAKTOHIMFIRSTTOFINDOUTWHATHISEXPERIENCEWAS“UEFAHASOPENEDDISCIPLINARYPROCEEDINGSAGAINSTCSKAFORTHE“RACISTBEHAVIOUROFTHEIRFANS“DURINGCITYS21WINMICHELPLATINI,PRESIDENTOFEUROPEANFOOTBALLSGOVERNINGBODY,HASALSOORDEREDANIMMEDIATEINVESTIGATIONINTOTHEREFEREESACTIONSCSKASAIDTHEYWERE“SURPRISEDANDDISAPPOINTED“BYTOURESCOMPLAINTINASTATEMENTTHERUSSIANSIDEADDED“WEFOUNDNORACISTINSULTSFROMFANSOFCSKA“AGEHASREACHEDTHEENDOFTHEBEGINNINGOFAWORDMAYBEGUILTYINHISSEEMSTOPASS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)總是逃避以為否認(rèn)不安情緒的存在就能萬事大吉，可這也恰好妨礙了我們經(jīng)歷最需要的生活體驗。下次感到不安時，不管有多害怕，也請試著勇敢面對吧。3ASKYOURHEARTWHATITWANTS傾聽內(nèi)心WEREOFTENCONFUSEDATTHENEXTSTEPTOTAKE,MAKINGPROSANDCONSLISTSUNTILOUREYESBLEEDANDOURBRAINSARESOREINSTEADOFALWAYSTAKINGTHISAPPROACH,WHATIFWEENGAGEDANEWPARTOFOURSELVESTHATISNTUSUALLYINVOLVEDINTHEDECISIONMAKINGPROCESS我們常對未來猶疑不定，反復(fù)考慮利弊直到身心俱疲。與其一味顧慮重重，不如從局外人的角度看待決策之事。FROMHTTP/WWWHXENCOM/ENGLISHARTICLE/YINGYUMEIWEN/20130806/227968HTMLTHESUPERSONICDESCENTCOULDHAPPENASEARLYASSUNDATHEWEATHETHEBALLOONWILLSLOWLYDRIFTTOTHEED