血培養(yǎng)陽性標本直接細菌鑒定和藥敏分析.doc

血培養(yǎng)陽性標本直接細菌鑒定和藥敏分析 李燕 日照市東港區(qū)人民醫(yī)院檢驗科，山東日照276800 摘要目的對血培養(yǎng)陽性標本直接細菌鑒定和藥敏進行分析。方法研究我院收治的發(fā)熱合并全身感染患者86例，在專用接種水中混摻患者的血培養(yǎng)標本，并在鑒定板上接種，通過直接法鑒定，同時把陽性標本轉種平板進行分離培養(yǎng)，根據(jù)常規(guī)法對分純后的菌落實施上機鑒定及藥敏實驗，然后進行對比。結果在86例樣品當中，直接法和常規(guī)法符合率：細菌鑒定總符合率為96.4%，其中革蘭陰性菌95.8%，革蘭陽性球菌97.1%；兩種方法的細菌結果均有著較高的準確率，細菌鑒定準確率在95.7%以上。結論血培養(yǎng)陽性標本直接細菌鑒定和藥敏實驗能夠準確的早期診斷菌血癥患者，值得推廣。 關鍵詞血培養(yǎng)；陽性標本；細菌鑒定；藥敏實驗 R446A1672-5654（xx）05（c）-0001-02 Directbacterialidentificationandsusceptibilityanalysisofpositivebloodculturespecimens LIYan DepartmentofDonggangRizhaoCityDistrictPeoplesHospital，Rizhao276800,China AbstractObjectiveDirectlyonpositivebloodculturespecimenswereanalyzedbacterialidentificationandsusceptibility.MethodsFeverbinedstudyofsystemicinfectioninourhospital86patientsvainatedinspecialwaterblendingbloodculturespecimensofpatients,andintheidentificationofinoculationplate,identifiedbythedirectmethod,whilethetransferofpositivesampleswereisolatedandculturedspeciestablet,aordingtoconventionalActonthecoloniesaftertheimplementationofapuremachineidentificationandsusceptibilitytesting,andthenpared.ResultsIn86samples,methodfortheidentificationofsusceptibilitydirectlywiththerateof96.4%,thetestresultsofP0.05,nostatisticalsignificance;theresultsofbacteriaidentificationaurac