安醫(yī)大生殖醫(yī)學(xué)課件03精子發(fā)生的分子機制

精子發(fā)生的分子機制生精波精子發(fā)生spermatogenesis精子變形減數(shù)分裂有絲分裂Thenucleusiscolouredinblue.PA,proacrosomicgranules;AV,acrosomicvesicle;GA,Golgiapparatus;AC,acrosomiccap;MAN,manchette;Cl,cytoplasmiclobeReproductiveDisorders—WhatWeHaveLearnedfromMutationsinSteroidogenesis(J.LarryJameson,MD,PhD)P-192REPRODUCTIVEOUTCOMEOFOOCYTEACTIVATIONBYCALCIUMIONOPHOREA23187FOLLOWINGINTRACYTOPLASMICSPERMINJECTION(ICSI)INPATIENTSWITHPREVIOUSCOMPLETEFERTILIZATIONFAILURE.T.Caballero,P.J.Buzzi,M.P.Zappacosta,A.Valcarcel,F.Lorenzo.IFERInstitutodeGinecologíayFertilidad,BuenosAires,Argentina.O-294EFFECTOFSPERMMATURITYANDASSISTEDOOCYTEACTIVATIONONMORPHOKINETICSOFEARLYHUMANEMBRYOS.T.Takeuchi,Y.Mori,Y.Nakajo,N.Aono1,T.Okuda,K.Kyono.KyonoARTClinicTakanawa,Minatoku,Tokyo,Japan;KyonoARTClinic,Sendai,Miyagi,Japan.ArrestofspermatogenesisatroundspermatidsinPLCz1deficientmiceM.Ito1,K.Nagaoka2,K.Kuroda3,N.Kawano4,K.Yoshida4,T.Shikano5,Y.Harada4,M.Miyado4,S.Oda6,K.Toshimori7,S.Miyazaki5,K.Miyado4,T.Suzuki11.HamamatsuUniversitySchoolofMedicine,Shizuoka2.TokyoUniversityofAgricultureandTechnology,Tokyo3.JuntendoUniversitySchoolofMedicine,Tokyo4.NationalCenterforChildHealthandDevelopment,Tokyo5.TokyoWomen’sMedicalUniversityofMedicine,Tokyo6.UniversityofTokyo,Chiba7.ChibaUniversity,Chiba,Japan1、LossofactivitymutationsinphospholipaseC

zeta(PLCζ)abolishescalciumoscillatoryabilityofhumanrecombinantproteininmouseoocytes.H398PKashirJ,JonesC,LeeHC,RietdorfK,NikiforakiD,DurransC,RuasM,TeeST,HeindryckxB,GalioneA,DeSutterP,FissoreRA,ParringtonJ,CowardK.HumReprod.2011Dec;26(12):3372-87.2、AmaternallyinheritedautosomalpointmutationinhumanphospholipaseC

zeta(PLCζ)leadstomaleinfertility.H233LKashirJ,KonstantinidisM,JonesC,LemmonB,LeeHC,HamerR,HeindryckxB,DeaneCM,DeSutterP,FissoreRA,ParringtonJ,WellsD,CowardK.HumReprod.2012Jan;27(1):222-31.Epub2011Nov16.3、CharacterizationoftwoheterozygousmutationsoftheoocyteactivationfactorphospholipaseC

zeta(PLCζ)fromaninfertilemanbyuseofminisequencingofindividualspermandexpressioninsomaticcells.KashirJ,KonstantinidisM,JonesC,HeindryckxB,DeSutterP,ParringtonJ,WellsD,CowardK.FertilSteril.2012Aug;98(2):423-31.

Sperm-derivedWWdomain-bindingprotein,PAWP,elicitscalciumoscillationsandoocyteactivationinhumansandmice.AarabiM,BalakierH,BasharS,MoskovtsevSI,SutovskyP,LibrachCL,OkoR.FASEBJ.2014Oct;28(10):4434-40.doi:10.1096/fj.14-256495.Epub2014Jun26.

Sperm-specificpost-acrosomalWW-domainbindingprotein(PAWP)doesnotcauseCa2+releaseinmouseoocytesNomikosM,SandersJR,TheodoridouM,KashirJ,MatthewsE,NounesisG,LaiFA,SwannK.MolHumReprod.2014Oct;20(10):938-47.doi:10.1093/molehr/gau056.Epub2014Jul23.SpermFactorsandOocyteActivation:CurrentControversiesandConsiderationsAmdaniSN,YesteM,JonesC,CowardK.BiolReprod.2015Aug;93(2):50SpermPostacrosomalWWDomain-BindingProteinIsNotRequiredforMouseEggActivation.SatouhY,Noz