蠲痹顆粒對膠原誘導(dǎo)性關(guān)節(jié)炎小鼠Th17-Treg細胞平衡調(diào)控作用探究

1、蠲痹顆粒對膠原誘導(dǎo)性關(guān)節(jié)炎小鼠thl7/treg細胞平衡調(diào)控作用探究【摘要】目的：研究蠲痹顆粒對膠原誘導(dǎo)性小鼠關(guān) 節(jié)炎thl7/treg細胞平衡調(diào)控作用，doi： 10. 3969/j. issn. 2095-4174. 2014. 04.008study on the balanced regulation of juanbi granule on thl7/treg cells in mice with collagen-induced arthritisliu wei-chao, li ling-yu, wan chun-ping, li zhao-fu, zheng xi, qi ya

2、n, wu jing-jin, peng jiang-yunabstract 】 objective : to study the balanced regulation of juanbi granule on thl7/treg cells in mice with collagen-induced arthritis in order to explore its mechanism in the treatment of rheumatoid arthritismethods： the arthritis models were induced by the bovine type i

3、i collagen. 20 dba/1 mice were divided equally into the cia group and the juanbi granule group according to the clinical score .the arthritis clinical score met hod was used to observe the serious ness of the on set of arthritis in cia mice ； the flow cytometry was used to detect the subpopula.tion

4、of the splenic lymphocytes (cd4, cd8, b220), and the thl7, thl cytokines and the expression of treg cells in cd4+t cells.results : juanbi granule could significantly reduce the clinical score of cia mice with collagen-induced arthr it is, but had no signif ica nt effect on the subpopulation of the s

5、plenic lymphocytes (cd4, cd8, b220) .meanwhile, it down regulated the expression level of il-17a in the cd4+t cells of cia mice, promoting the expression of treg cells in a certain extent.conclusion： regulating the balance of the thl7 cells and treg cells and down-regulating the expression of the pr

6、oinflammatory cytokine il-17a may be one of its mechanisms in the treatment of rheumatoid arthritis.keywords arthritis, rheumatoid； arthritis, collagen-induced； juanbi granule； th 17 cell； regulatory t cells； mice蠲痹顆粒是中醫(yī)學家吳佩衡學術(shù)繼承人、云南省名老中 醫(yī)吳生元教授基于扶陽理論總結(jié)出的治療類風濕關(guān)節(jié)炎 (rheumatoid arthritis, ra)的效方,主要由附片、川

7、葦、 赤芍、桂枝、麻黃、蕙該仁、五加皮等組成，具有溫經(jīng)散寒、 祛風除濕、通絡(luò)止痛之功，主治寒濕痹阻之ra。最新研究表 明，treg. th 17細胞功能和分化過程具有共同之處卻又相互 對抗，其平衡失調(diào)影響著ra發(fā)生與轉(zhuǎn)歸1-2 o1實驗材料5參考文獻1 littman dr, rudensky ay. th17 and regulatory t cells inmediatingandrestraininginflammationj. cell, 2010, 140 (6)： 845858.2 bettelli e , carrier y , gao w. reciprocal develop

8、mental pathways for the generation of pathogenic effector th17 and regulatory t cellsj. nature, 2006, 441 (7090)： 235-2383 萬春平，彭江云，李兆福，等蠲痹顆粒對膠原誘 導(dǎo)性小鼠關(guān)節(jié)炎的抑制作用及機制研究j.中藥材，2013, 36 (9)： 1505-1507.4 周茹，楊以阜，左建平.ii型膠原誘導(dǎo)的小鼠關(guān)節(jié) 炎動物模型的建立及影響因素j.中國藥理學通報，2006, 22 (12)： 1532-1535.5 king c, tangye sg, mackay cr. t follicular he